Saliva Contamination by Opportunistic Microorganisms in Drug Addiction Females
Resumo
In recent decades there has been a significant increase in the consumption of legal and illegal drugs, and most of such compounds are able to induce dependence and this increase was observed mainly in females. This drug addiction increases susceptibility to several infectious agents, especially opportunistic microorganisms. The objective of this study was to evaluate the occurrence of opportunistic bacteria and yeasts in the mouth of drug addiction patients and non-addicted patients with different periodontal conditions. The study included 50 addiction patients and 200 non-addiction subjects. Intra and extraoral clinical examinations were performed and saliva samples were transferred to saline solution and the presence of members of the family Enterobacteriaceae, genera Enterococcus and Pseudomonas, as well fungi of the genus Candida was evaluated by culture. Samples were cultivated onto selective and non-selective media under aerobic conditions, at 37oC, for 24 -48 h. Identification of selected microorganisms were carried out through biochemical tests. Chi-square test was used to evaluate the data when three or more categories were involved. Higher detection frequencies of Candida species, family Enterobacteriaceae, E. faecalis, Pseudomonas sp. and P. aeruginosa in addiction patients were verified. It was found that patients addicted to both genders showed a higher occurrence of members of the Enterobacteriaceae, which were also associated with bone loss only in patients with drug addiction.Keywords: Drugs, Pathogens, Contamination, Prevention.
Downloads
Referências
Duailibi LB, Ribeiro M, Laranjeira K. Profile of cocaine and crack users in Brazil. Cad Saúde Pública. 2008; 24(Suppl 4): 545-57.
Guindalini C, Vallada H, Breen G, Laranjeira R. Concurrent crack and powder cocaine users from Sao Paulo: Do they represent a different group. BMC Public Health. 2006; 6: 10 doi:10.1186/1471-2458-6-10.
Waldschmidt TJ, Cook RT, Kovacs EJ. Alcohol and inflammation & immune responses: summary of the 2006 alcohol and immunology research interest group (AIRIG) meeting. Alcohol. 2008; 42(2): 137-42.
Heinz R, Waltenbaugh C. Ethanol-consumption modifies dendritic cell antigen presentation in mice. Alcohol Clin Exp Res. 2007; 31: 1759–71.
Irwin MR, Wang M, Valladares EM, Motivala SJ, Fong T, Newton T, et al. Cocaine dependence and acute cocaine induce decreases of monocyte proinflammatory cytokine expression across the diurnal period: autonomic mechanisms. J Pharmacol Experiment Ther. 2007; 320: 507-15.
Jaffe JA, Kimmel PL. Chronic nephropathies of cocaine and heroin abuse: a critical review. Clin J Am Soc Nephrol. 2006; 1: 655-67.
Pieper B, Hopper JA. Injection drug use and wound care. Nurs Clin N Am. 2005; 40: 349-63.
Brazier WJ, Dhariwal DK, Patton DW, Boshop K. Ecstasy related periodontitis and mucosal ulceration: a case report. Br Dent J. 2003; 194: 197-9.
Amaral CSF, Luiz RR, Leão ATT. The relationship between alcohol dependence and periodontal disease. J Periodontol. 2008; 79: 993-8.
Brand HS, Gonggrijp S, Blanksma CJ. Cocaine and oral health. Br Dent J. 2008; 204: 365-9.
Glaser R; Kiecolt-Glaser JK. Stress-induced immune dysfunction: implications for health. Nature Rev. 2005; 5(3): 243-51.
Zambon JJ, Grossi SG, Machtei EE, Ho AW, Dunford R, Genco RJ. Cigarette smoking increases the risk for subgingival infection with periodontal pathogens. J Periodontol. 1996; 67(Suppl 10): 1050-4.
Lafaurie GI, Contreras A, Barón A, Botero J, Mayorga-Fayad I, Jaramillo A, et al. Demographic, clinical and microbial aspects of chronic and aggressive periodontitis in Colombia: a multicenter study. J Periodontol. 2007; 78: 629-39.
Herrera D, Contreras A, Gamonal J, Oteo A, Jaramillo A, Silva N, et al. Subgingival microbial profiles in chronic periodontitis patients from Chile, Colombia and Spain. J Clin Periodontol. 2008; 35: 106-13.
Corraini P, Baelum V, Pannuti CM, Pustiglioni AN, Romito GA, Pustiglioni FE. Periodontal attachment loss in an untreated isolated population of Brazil. J Periodontol. 2008; 79: 610-20.
Armitage G. Periodontal diagnoses and classification of periodontal diseases. Periodontol 2000. 2004; 34: 9-21.
Gaetti-Jardim Junior E, Nakano V, Wahasugui TC, Cabral FC, Gamba R, Avila-Campos MJ. Occurrence of yeasts, enterococci and other enteric bacteria in subgingival biofilm of HIV-positive patients with chronic gingivitis and necrotizing periodontitis. Braz J Microbiol. 2008; 39(2): 257-61.
Shimada MH, Ciesielsky FIN, Gaetti-Jardim EC, Gaetti-Jardim Junior E. Emprego de saliva na determinação do risco às doenças periodontais: aspectos microbiológicos e clínicos. Rev Odontol UNESP. 2008; 37: 183-9.
Price RR, Viscount HB, Stanley MC, Leung K-P. Targeted profiling of oral bacteria in human saliva and in vitro biofilms with quantitative real-time PCR. Biofouling. 2007; 23(3-4): 203-13.
Gontijo B, Bittencourt FV, Lourenço LFS. Skin manifestations of illicit drug use. An Bras Dermatol. 2006; 81: 307-17.
Slots J, Rams TE, Feik D, Taveras HD, Gillespie GM. Subgingival microflora of advanced periodontitis in the Dominican Republic. J Periodontol. 1991; 62: 543-7.
Persson GR, Hitti J, Paul K, Hirschi R, Weibel M, Rothen M, et al. Tannerella forsythia and Pseudomonas aeruginosa in subgingival bacterial samples from parous women. J Periodontol. 2008; 79: 508-16.
Botero, JE, Contreras A, Lafaurie G, Jaramillo A, Betancourt M, Arce RM. Occurrence of periodontopathic and superinfecting bacteria in chronic and aggressive periodontitis subjects in a Colombian population. J Periodontol. 2007; 78: 696-704.
Eick S, Pietkiewicz M, Sculean A. Oral microbiota in Swiss adolescents. Clin Oral Invest. 2013; 17(1): 76-86.
Samaranayake LP, Leung WK, Jin L. Oral mucosal fungal infections. Periodontol 2000. 2000; 49: 39-59.
Gaetti-Jardim Junior E, Ciesielski FIN, Sousa FRN, Nwaokorie F, Schweitzer CM, Avila-Campos MJ. Occurrence of yeasts, pseudomonads and enteric bacteria in the oral cavity of patients undergoing head and neck radiotherapy. Braz J Microbiol. 2011; 42: 1047-55.
Jham BC, França EC, Oliveira RR, Santos VR, Kowalski LP, Freire ARS. Candida oral colonization and infection in Brazilian patients undergoing head and neck radiotherapy: a pilot study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007; 103: 355-8.
Dahlén G. Non-odontogenic infections in dentistry. Periodontol 2000. 2010; 49: 7-12.
Gordon RJ, Lowy FD. Current concepts: bacterial infections in drug users. New Engl J Med. 2005; 353: 1945-54.
Diz Dios P, Feijoo J, Alvarez FJ, Castro M, Varela J. Oral enterobacteriaceae in HIV patients. I International Conference of AIDS; 1993. Berlin; 1993. p.436.
Fuentefria DB, Ferreira AE, Graf T. Corção G. Pseudomonas aeruginosa: disseminação de resistência antimicrobiana em efluente hospitalar e água superficial. Rev Soc Bras Med Trop. 2008; 41: 470-3.