Chemical, Morphological and Bacterial Adhesion Analysis of Orthodontic Wires Composed of Different Metallic Alloys

Authors

  • Maria Leticia Lopes Miguel Braga Department of Orthodontics, Araras Dental School, Herminio Ometto Foundation, FHO, 13607-339 Araras - SP, Brazil https://orcid.org/0009-0007-7806-1339
  • Analia Gabriella Borges Ferraz-Facury Department of Restorative Dentistry, Dental Materials Division, Piracicaba Dental School, Unicamp, 13414-903 Piracicaba - SP, Brazil https://orcid.org/0000-0001-9255-3937
  • Eduardo Martinelli Franco Department of Biosciences, Piracicaba Dental School, Unicamp, 13414-903 Piracicaba - SP, Brazil https://orcid.org/0000-0001-5392-0167
  • Larissa Marcelino Department of Restorative Dentistry, Dental Materials Division, Piracicaba Dental School, Unicamp, 13414-903 Piracicaba - SP, Brazil https://orcid.org/0000-0002-7199-9013
  • Luan dos Santos Menezes Department of Restorative Dentistry, Dental Materials Division, Piracicaba Dental School, Unicamp, 13414-903 Piracicaba - SP, Brazil https://orcid.org/0000-0002-4656-2848
  • Ana Rosa Costa School of Dentistry of Piracicaba (UNICAMP), Department of Dental Materials, 13414-903 Piracicaba - SP, Brazil/Department of Orthodontics, Araras Dental School, Herminio Ometto Foundation, FHO, 13607-339 Araras - SP, Brazil https://orcid.org/0000-0002-1020-3210
  • José Guilherme Neves School of Dentistry of Piracicaba (UNICAMP), Department of Dental Materials, 13414-903 Piracicaba - SP, Brazil/ Department of Orthodontics, Araras Dental School, Herminio Ometto Foundation, FHO, 13607-339 Araras - SP, Brazil https://orcid.org/0000-0003-1613-8496

DOI:

https://doi.org/10.21270/archi.v12i9.6256

Keywords:

Alloys, Orthodontics, Streptococcus mutans, Wire

Abstract

The purpose of this study was to evaluate the chemical, morphological and bacterial adhesion characteristics of orthodontic arches composed of different metal alloys. The wire segments (n=10) were allocated to the following groups: G1) Tru-Chrome- Rock Mountain Steel Wire (Colorado-USA); G2) NiTi- Rock Mountain Wire (Colorado-USA); G3) TiMb Rock Mountain Wire (Colored-USA); G4) NbTi Gummetal- Rock Mountain Wire (Colorado-USA). The orthodontic arches were segmented (20mm) and sterilized by means of ultraviolet light. Using Confocal Laser Microscopy (CLM), the roughness of archwires were investigated. The metallic alloys composition was analyzed by means of Scanning Electron Microscopy/Energy-dispersive Spectroscopy (SEM/EDS). The S. mutans biofilm growth was performed on the arches and analyzed by means of SEM and Spectrophotometry. Surface roughness and Spectrophotometry data were submitted to one-way ANOVA, followed by Tukey's test (α=0.05) and SEM/EDS data obtained by exploratory analysis. The NbTi arches showed higher surface roughness when compared to the other groups, followed by the NiTi and TiMb arches and the steel arches group (p<0.05). It was observed a lower adherence of S. mutans biofilm in steel arches when compared to the other groups. The absorbance results showed higher biofilm formation for the NbTi group, followed by Steel, NiTi and TiMb. (p<0.05). The results of EDS confirm the compositions proposed by the manufacturer. It was concluded that the alloy type in orthodontic arches has an effect on surface roughness. The chemical and morphological characteristics of the arches are related to the adhesion of S. mutans biofilm.

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References

Millett DT, Mandall NA, Mattick RC, Hickman J, Glenny AM. Adhesives for bonded molar tubes during fixed brace treatment. Cochrane Database Syst Rev. 2017;23(2):CD008236.

Eliades T. Orthodontic material applications over the past century: Evolution of research methods to address clinical queries. Am J Orthod Dentofacial Orthop. 2015;147(5):224231.

McLaughlina RP,Bennett. JC Evolution of treatment mechanics and contemporary appliance design in orthodontics: A 40-year perspective, Am J Orthod Dentofacial Orthop. 2015;147:654-62.

Philippe J. La naissance de l’Edgewise ou le dernier et le meilleur mécanisme d’Angle, Orthod. 2016;87:347–351. French.

Abbate GM, Caria MP, Montanari P, Mannu C, Orrù G, Caprioglio A, Levrini L. Periodontal health in teenagers treated with removable aligners and fixed orthodontic appliances, J Orofac Orthop. 2015;76(3):240-50.

Dos Santos AA, Pithon MM, Carlo FG, Carlo HL, de Lima BA, Dos Passos TA, Lacerda-Santos R. Effect of time and pH on physical-chemical properties of orthodontic brackets and wires. Angle Orthod. 2015;85(2):298-304

Fatani EJ, Almutairi HH, Alharbi AO, Alnakhli YO, Divakar DD, Muzaheed, et all. In vitro assessment of stainless steel orthodontic brackets coated with titanium oxide mixed Ag for anti-adherent and antibacterial properties against Streptococcus mutans and Porphyromonas gingivalis. Microbial Pathogenesis Microb Pathog. 2017;112:190-194.

Al-Anezi AS. The effect of orthodontic bands or tubes upon periodontal status during the initial phase of orthodontic treatment. Saudi Dent J. 2015;27(3):120- 4.

Mártha K, Lőrinczi L, Bică C, Gyergyay R, Petcu B, Lazăr L. Assessment of Periodontopathogens in Subgingival Biofilm of Banded and Bonded Molars in Early Phase of Fixed Orthodontic Treatment. Acta Microbiol Immunol Hung. 2016;63(1):103-13.

Chin MYH, Busscher HJ, Evans R, Noar J, Pratten J Early biofilm formation and the effects of antimicrobial agents on orthodontic bonding materials in a parallel plate flow chambre, Eur J Orthod. 2006;28(1):1–7.

Mhaske AR, Shetty PC, Bhat NS, Ramachandra CS, Laxmikanth SM, Nagarahalli K, et al. Antiadherent and antibacterial properties of stainless steel and NiTi orthodonic wires coated with silver against Lactobacillus acidophilus—an in vitro study. Prog Orthod. 2015:16:40

Dias AP, Paschoal MAB, Diniz RS, Lage LM, Gonçalves LM. Antimicrobial action of chlorhexidine digluconate in self-ligating and conventional metal bracketsinfected with Streptococcus mutans biofilm, Clin Cosmet Investig Dent. 2018:10 69-74.

Altmann AS, Collares FM, Leitune VC, Arthur RA, Takimi AS, Samuel SM, In vitro antibacterial and remineralizing effect of adhesive containing triazine and niobium pentoxide phosphate inverted glass, Clin Oral Invest. 2017;21(1):93-103.

Heymnn GC, Grauer D, A Contemporary review of white spot lesions in orthodontics. J Esthet Restor Dent. 2013;25(2):85-95.

Korkmaz YN, Yagci A, Comparing the effects of three different fluoride-releasing agents on white spot lesion preventionin patients treated with full coverage rapid maxillary expanders, Clin Oral Investig. 2019;23(8):3275-285.

Alavi S,Yaraghi N, The effect of fluoride varnish and chlorhexidine gel on white spots and gingival and plaque indices in fixed orthodontic patients: A placebo- controlled study, Dent Res J (Isfahan). 2018;15(4):276-82.

Murakami T, Iijima M, Muguruma T, Yano F, Kawashima I, Mizoguchi I. High- cycle fatigue behavior of beta-titanium orthodontic wires. Dent Mater J. 2015;34(2):189-95.

Biesiekierski A, Lin J, Munir K, Ozan S, Li Y, Wen C. An investigation of the mechanical and microstructural evolution of a TiNbZr alloy with varied ageing time. Sci Rep. 2018;8(1):5737.

Degrazia FW, Altmann ASP, Ferreira CJ, Arthur RA, Leitune VCB, Samuel SMW et al. Evaluation of an antibacterial orthodontic adhesive incorporated with niobium-based bioglass: an in situ study. Braz Oral Res. 2019;33:e010.

Dux KE. Implantable Materials Update. Clin P Med Surg. 2019;36:535-42.

Sepúlveda CH, Gontijo SML, Santos LA, Drummond AF, Menezes LFS, Neves LS et al. Influence of heat treatment on the mechanical properties of CrNi stainless steel orthodontic wires. Dental Press J Orthod. 2019;24(1):68-73.

Kuntz ML, Vadori R, Khan MI. Review of Superelastic Differential Force Archwires for Producing Ideal Orthodontic Forces: an Advanced Technology Potentially Applicable to Orthognathic Surgery and Orthopedics. Curr Osteoporos Rep. 2018;16(4):380-86.

Rincic Mlinaric M, Karlovic S, Ciganj Z, Acev DP, Pavlic A, Spalj S. Oral antiseptics and nickel-titanium alloys: mechanical and chemical effects of interaction. Odontology. 2019;107(2):150-57.

Asri RIM, Harun WSW, Samykano M, Lah NAC, Ghani SAC, Tarlochan F, Raza MR. Corrosion and surface modification on biocompatible metals: A review. Mater Sci Eng C Mater Biol Appl. 2017;77:1261-274.

Kaur M, Singh K. Review on titanium and titanium based alloys as biomaterials for orthopaedic applications. Mater Sci Eng C Mater Biol Appl. 2019;102:844-62.

Moraes JJ, Stipp RN, Harth-Chu EN, Camargo TM, Höfling JF, Mattos-Graner RO. Two-component system VicRK regulates functions associated with establishment of Streptococcus sanguinis in biofilms. Infect Immun. 2014;82(12):4941-51.

Rafiee K, Naffakh-Moosavy H, Tamjid E. The effect of laser frequency on roughness, microstructure, cell viability and attachment of Ti6Al4V alloy. Mater Sci Eng C Mater Biol Appl. 2020;109:110637.

Inami T, Tanimoto Y, Yamaguchi M, Shibata Y, Nishiyama N, Kasai K. Surface topography, hardness, and frictional properties of GFRP for esthetic orthodontic wires. J Biomed Mater Res B Appl Biomater. 2016;104(1):88-95.

Vincent M, Duval RE, Hartemann P, Engels-Deutsch M. Contact killing and antimicrobial properties of copper. J Appl Microbiol. 2018;124(5):1032-46.

Hans M, Mathews S, Mücklich F, Solioz M. Physicochemical properties of copper important for its antibacterial activity and development of a unified model. Biointerphases. 2015;11(1):018902.

Tang S, Zheng J. Antibacterial Activity of Silver Nanoparticles: Structural Effects. Adv Healthc Mater. 2018;7(13):e1701503.

Abraham KS, Jagdish N, Kailasam V, Padmanabhan S. Streptococcus mutans adhesion on nickel titanium (NiTi) and copper-NiTi archwires: A comparative prospective clinical study. Angle Orthod. 2017;87(3):448-54.

Taha M, El-Fallal A, Degla H. In vitro and in vivo biofilm adhesion to esthetic coated arch wires and its correlation with surface roughness. Angle Orthod. 2016;86(2):285-91.

Kim IH, Park HS, Kim YK, Kim KH, Kwon TY. Comparative short-term in vitro analysis of mutans streptococci adhesion on esthetic, nickel-titanium and stainless steel arch wires. Angle Orthod. 2014; 84:680–686

Macena MCB, Sá Catão CD, Rodrigues RQF, Vieira JMF. Orthodontic Wires, Microstructural Properties and Their Clinical Applications: Overview Rev Saúde & Ciência On-line. 2015;4(2):90-108.

Published

2023-09-14

How to Cite

Braga, M. L. L. M., Ferraz-Facury, A. G. B., Franco, E. M., Marcelino, L., Menezes, L. dos S., Costa, A. R., & Neves, J. G. (2023). Chemical, Morphological and Bacterial Adhesion Analysis of Orthodontic Wires Composed of Different Metallic Alloys. ARCHIVES OF HEALTH INVESTIGATION, 12(9), 1962–1968. https://doi.org/10.21270/archi.v12i9.6256

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Original Articles